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Glutathione synthetase deficiency has been incriminated as the cause of chronic hemolytic anemia alone (due to isolated RBC enzyme deficiency) and as the cause of a generalized syndrome (due to enzyme deficiency in many tissues) characterized by mild hemolytic disease, severe metabolic acidosis, and mental deterioration

concentration of this substrate in the initial, uninoculated, culture medium is typically greater than about 2% w/v, more typically between about 2% and 10% w/v, more typically between about 3% and 8% w/v

In contrast, non-heme iron is found predominantly in an insoluble form as Fe 3+