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5 The Lancet CagriSema weight-loss outcomesReported mean body-weight reduction (~20%) with the branded CagriSema pharmaceutical in controlled trials

After the centrifuge, digestion was performed in 0.05% trypsin-EDTA (T4174, Sigma-Aldrich, MO, USA) for 5 min at 37 C and stopped by adding an equal volume of trypsin inhibitor (T6522, Sigma-Aldrich) in phosphate-buffered saline (PBS)

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For control, the calibration set consists of the [GSH] and [GSSG] measurements at PN11, PN13 and PN15, and the validation set contains the data at PN15, PN17 and PN28