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2.13 DHE stain For human umbilical vein endothelial cells (HUVECs), after different treatment groups, the cells were cultured in a glucose-free medium under hypoxic conditions for 3 h

Antioxidant kinetics in lung lavage fluid following exposure of humans to nitrogen dioxide

10.3390/nu15234852 Nutrients 103 PalmerJ

Each sample was then split into two fraction (100 L) and treated as follows: For H 2 S measurement, 100 L of each sample was precipitated with 1 mL of absolute MeOH at 40 C for 2 h, centrifuged (16,000 g at 0 C), and obtained supernatant was evaporated and the residue was resuspended and sonicated in 100 L of 50 mM HEPES buffer (pH 7.4)