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Feng, SY et al

Disrupted copper metabolism may become a potential factor in the worsening of neurodegenerative diseases and cerebral ischemic injuries, suggesting that special attention should be given to copper balance in the treatment of stroke and other neuroimmune diseases (227)

After cell detachment, the cells were counted and frozen at 3.0 10 5 cells/250 L in 90% FBS, 5% DMSO, and 5 mM GSH (Teva Takeda Pharma Ltd., Aichi, Japan)