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Conjugation of the thiol group of glutathione to the CDNB substrate was monitored by recording changes in absorbance at 340 nm between 0.5 and 6 min after initiating the reaction at 22C

Moreover, another study by Sagrillo-Fagundes and his group found that melatonin significantly regulated autophagy and inflammation in primary trophoblast cells that were exposed to a hypoxic/reoxygenation environment, mimicking preeclamptic pregnancy, thus reducing apoptosis in these cells

doi: Emmez H, Yildirim Z, Kale A, Tnge M, Durda E, Brcek A, Uanku LN, Doulu F, Kili N, Baykaner MK (2010) Anti-apoptotic and neuroprotective effects of alpha-lipoic acid on spinal cord ischemiareperfusion injury in rabbits

Limit test studies failed to establish an LD50, indicating exceptionally low acute toxicity even at very high doses