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Live imaging microscopy of adipocytes transduced with an Akt sensor (FoxO1-Clover) demonstrated that FoxO1 translocation from the nucleus to the cytosol was reduced, indicting less Akt activation in Mg 2+ -deficient adipocytes

4.13.2 Mechanical stress Mechanical testing demonstrates increased tissue stiffness that limits adipocyte expansion and triggers mechanotransduction through focal adhesion complexes (327, 328)

This is particularly intriguing, as the recent work uncovered that U1-snRNP may serve as a governing machinery for blocking premature polyadenylation 48 , suggesting FUS/TLS might play an important role in regulating polyadenylation selection

2.12 Bodipy (581/591)-C11 and MitoTracker deep red FM staining Bodipy (581/591)-C11 (Invitrogen, U.S.A.) and MitoTracker Deep Red FM were used to detect lipid peroxidation and mitochondrial localization, respectively