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The resulting systems were prepared for simulations using in-house scripts based on HTMD2.3.2 45 and VMD1.9.4 frameworks

Mutants selected with pyrimethamine (input 2) were then used to co-infect mice with a WT parasite line that constitutively expressed Cas9 under an hsp70 promoter ( cas9 )

Disruptions in iron metabolism leads to the accumulation of intracellular free iron, which catalyzes the generation of reactive oxygen species (ROS) through the Fenton reaction