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Detection of GSTP1 metabolically labelled with -alkynyl-palmitate using click chemistry Immunoprecipitated GSTP1 was eluted from the agarose beads conjugated to mouse anti-V5 antibody (V5-10), by incubating at 80C for 15 min in 50 mM HEPES, pH 7.4, with 1% SDS

During a subsequent 24-week extension period, the ritlecitinib groups continued with their maintenance doses of 50mg, 30mg, or 10mg once daily, and patients initially assigned to placebo were switched to ritlecitinib 50mg once daily with or without a 4-week 200mg loading dose regimen

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