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In bacterial and fungal cells, 3BP has been shown to inhibit also isocitrate lyase and malate synthase, which play a pivotal role in the glyoxylate cycle and are associated with virulence (Dunn et al., Recent studies on the interaction of 3BP with various enzymes, including glycolytic ones, have shown that the main cellular target for this compound is HK2 followed by PDH, and SDH (Yadav et al., 2017)

The buffer also included 1 M MLS-0038949 (in MeOH) to inhibit residual TNAP activity

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After adding laccase, the solution is stirred at a controlled temperature