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It also maintains other antioxidants, such as vitamins C and E, in their reduced active forms

The proteins were incubated in following buffer conditions: Binding buffer: 20 mM TrisHCl, 200 mM NaCl, 1 mM EDTA, pH 7.4 Elution buffer: 10 mM Maltose, 20 mM TrisHCl, 200 mM NaCl, 1 mM EDTA, pH 7.4 Post-elution, the pull-down eluates were subjected to SDS-PAGE analysis keeping only MBP as the control

About the study The clinical trial involved healthy adults aged 19 to 40, with a Body mass index (BMI) between 18.5 and 25 kg/m, who had consumed alcohol at least once a month and experienced hangover symptoms

e-GST assay represents a very simple, inexpensive, and fast laboratory tool in clinical nephrology also developed for high-throughput routine protocols 16,17