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Vitamin C protects against oxidative stress brought on by free radicals

For cell apoptosis assay, G422 cells were seeded in a 12-well plate at the density of 1 10 6 cells per well and incubated overnight

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The concentration of purified At LDOX was measured with a NanoDrop spectrophotometer (Thermo Fisher Scientific) at A 280nm using an extinction coefficient estimated with ProtParam 50