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The target gene expression was normalized to those of -actin, while the relative miRNA levels were normalized to the U6 or cel-miR-39 in sEV and calculated as 2 (CT)

Phylogenetic analysis Eukaryote orthologs of ADH5 were obtained from NCBI, CLUSTAL at phylogeny.fr was used to align the sequences, and TreeDyn at phylogeny.fr for tree generation 66