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Samples were heat retrieved at 95 C for 30 min in a pH 9 buffer (Agilent Technologies, Santa Clara, CA, USA, #S2367), cooled to 23 C and treated with 3% v/v H 2 O 2 in dH 2 O to block for endogenous peroxidase activity

Further findings showed that decreasing CAV2 in OSCC decreased BCL2 expression and increased c-JUN, suggesting that JUN activation may promote apoptosis by negatively regulating BCL2