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Bacterial lysis was performed by sonication on ice, and the lysates were clarified by centrifugation at 15,000 g for 30 min at 4C

68 Beyond hepatic processes, alterations in gut microbiota composition and impairment of the gut-liver barrier are key contributors to MASLD pathogenesis

European Journal of Clinical Nutrition 2013: Beyond weight loss: a review of the therapeutic uses of very low carbohydrate (ketogenic) diets Obesity Reviews 2014: Do ketogenic diets really suppress appetite