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Am J Reprod Immunol 43(4):202203 Toth B, Bohlmann M, Hancke K, Kuon R, Nawroth F, von Otte S, Rogenhofer N, Rudnik-Schoneborn S, Schleussner E, Tempfer C et al (2023) Recurrent miscarriage: diagnostic and therapeutic procedures

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[DOI] [PubMed] [Google Scholar] 46.Schiavi M.C., Di Tucci C., Colagiovanni V., Faiano P., Giannini A., DOria O., Prata G., Perniola G., Monti M., Zullo M.A., et al

The extract was rotary evaporated at 30 C to a volume of 2 ml, acidified to pH 4 with formic acid, and 200 l aliquots were purified using preparative HPLC (Agilent Technologies, 1260 Infinity) over a Luna 5 m phenyl-hexyl 100 (250 10 mm) column and a 15 min linear gradient from 0% acetonitrile in H 2 O to 16% acetonitrile in H 2 O at 4 ml min 1 flow