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4ml of blood was collected into an EDTA-Vacutainer tube, chilled on ice and centrifuged at 1500 g for 10 minutes at 4C

In addition, MAPKs, as the central mediators that transmit extracellular signals from cell membrane to nucleus, can regulate the activation of Nrf2 and NF-B pathways to reduce cell injuries (Pan et al., 2018)

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