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Sample sizes per experimental group were chosen based on previously reported similar studies [4, 5]

The solution was basified with 50 L of triethylamine and dialyzed (MW cut-off: 3500 Da) against methanol for 48 h, then dried under rotary evaporation to yield NH 2 -PEG 2k -PDHD

The study focused on eight thyroid traits, including TSH, FT4, and TPOAb levels, as well as four forms of thyroid dysfunction: subclinical hypothyroidism, isolated hypothyroxinemia, overt hyperthyroidism, and subclinical hyperthyroidism

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