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As the field advances toward personalized medicine, single-cell transcriptomics has revealed interindividual variability in neuronal glycolysis-oxidative phosphorylation balance (e.g., differential GLUT1/4 expression affecting substrate selection in APP/PS1 mice), offering new strategies for targeting AMPK-mediated astrocyte glycogenolysis to optimize neuronal energy supply (Wu et al., 2024)

The following parameters were modified compared to Watkins method: the chromatographic gradient, the column, and the mass spectrometer settings