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It had long been hypothesized that antioxidants might be able to protect against cancer because they neutralize reactive oxygen species (ROS) that can damage DNA

Interestingly, when we optimized the cell sorting procedure by using free settling to replace magnetic separation, the EGFP positive cell percentage could be elevated from 20% to over 95% in K-562 cells for both GST-EGFP-GPI DAF and GST-EGFP-GPI BY55 (Figure 3C)

Solubilized samples were centrifuged at 17,400 g for 30 min at 4 C, and 13.5 l of the supernatant was mixed with 1.5 l of 10 loading buffer (50% glycerol, 0.1% Ponceau S)