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Description

Stimulation by LPS and A induces microglial polarization towards the M1 phenotype, which involves significant reprogramming of glucose metabolism from OXPHOS to glycolysis, which is dependent on the mTOR-HIF-1 pathway

99 Wu et al

Accordingly, the present invention provides methods of generating monoclonal antibodies as well as antibodies produced by the method comprising culturing a hybridoma cell secreting an antibody of the invention wherein, preferably, the hybridoma is generated by fusing splenocytes isolated from a mouse immunized with an antigen of the invention with myeloma cells and then screening the hybridomas resulting from the fusion for hybridoma clones that secrete an antibody able to bind a polypeptide of the invention