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This sample size is consistent with prior mechanistic studies and the 3R principles [52, 53], although the relatively small cohort remains a limitation and should be expanded in future work

However, it exhibits low cell membrane permeability and demonstrates substantial toxicity in animal models, thereby constraining its potential application as a therapeutic agent (Zweygarth et al., 1986)

Efficacy and safety of metformin during pregnancy in women with gestational diabetes mellitus or polycystic ovary syndrome: a systematic review

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