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The quantity of LAB cells adhering to the cultured cells was determined using the methodology outlined by Gopal et al

The liquor is mixed by aeration devices (supplying also oxygen) A portion of the biological sludge separated from the secondary effluent by sedimentation is recycled to the aeration tank Types of AS Systems: Conventional, Complete-Mix, Sequencing Batch Reactor, Extended Aeration, Deep Tank, Deep Shaft Advantages Flexible, can adapt to minor pH, organic and temperature changes Small area required Degree of nitrification is controllable Relatively minor odor problems Disadvantages High operating costs (skilled labor, electricity, etc.) Generates solids requiring sludge disposal Some process alternatives are sensitive to shock loads and metallic or other poisons Requires continuous air supply Advantages/Disadvantages Trickling Filters The trickling filter or biofilter consists of a bed of permeable medium of either rock or plastic Microorganisms become attached to the media and form a biological layer or fixed film

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