The isolated BMDMs were finally resuspended and cultured in 5% CO 2 at 37 C with DMEM/F-12 complete medium containing M-CSF, 10% FBS, penicillin (50 U/mL) and streptomycin (100 mg/mL)
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Reconstitution Troubleshooting Quality Verification Checklist Before using reconstituted IGF-1 LR3 in experiments: [ ] Confirm lot certificate of analysis (HPLC purity 98%, mass spec identity) [ ] Document reconstitution date, solvent, concentration, volume [ ] Visual inspection: solution is clear and colorless [ ] Aliquots labeled with compound, concentration, date, freeze-thaw count [ ] First-use aliquot shows expected biological activity in pilot assay For information on what certificates of analysis should contain, see: Quality Testing and Third-Party Verification for IGF-1 LR3 Research Peptides