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Some researchers report using vials up to 28-30 days after reconstitution without noticeable potency loss, but the general consensus leans toward using the vial within 3 weeks for optimal results

GSH assay The relative GSH concentration in tissue lysates was assessed using a Glutathione Microplate Assay Kit (abs580006, Absin, Shanghai, China) according to the manufacturers instructions

Male Wistar rats weighing 170-190 g were pretreated with sodium molybdate, Na 2 MoO 4 (1.24 mmol/kg body weight, i.p., once a day) for 3 d and on day 4, they were exposed to N-nitrosodimethylamine (50 mg/kg body weight, once, i.p.)

doi: 10.1093/ajcn/53.4.1021S