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Description

Yang GY, Xu ZG, Peng SB, Sun YD, Jia CX, Zhai MZ

Endocannabinoid control of food intake and energy balance

To exclude the possibility of extracellular interactions between EPS and MPP + in vitro, we performed LCMS/MS analysis on the mixture of EPS and MPP +

2.7 Immunocytochemistry: preparation of Alexa Fluor-labeled proteins, cell surface binding, endocytosis, and downregulation of TfRs HemeHPX in PBS was fluorescently labeled using Alexa Fluor (AF) 546 or 647, according to the manufacturers instructions (Invitrogen)