glutathione s-transferase pull-down assays

glutathione s-transferase pull-down assays Pull down assay. Pull down assay was performed to study the interaction Transglutaminase 2 crosslinks the glutathione

Name: glutathione s-transferase pull-down assays Pull down assay. Pull down assay was performed to study the interaction Transglutaminase 2 crosslinks the glutathione
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Transglutaminase 2 crosslinks the glutathione S transferase tag, impeding proteinprotein interactions of the fused protein Experimental & Molecular Medicine Category:ECO:0001817 ! glutathione S transferase pull down assay evidence OMPwiki Purification of proteins fused to glutathione S tranferase PMC GST Pull Down Assay Protein Protein Interaction Using Pull Down Assay

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Description

The GSH biosynthesis has been described previously

Since then, plant GSTUs and GSTFs were found to bind several tetrapyrroles, e.g., protoporphyrin IX (Proto IX), Mg-protoporphyrin but also bacterial porphyrin derivatives identified upon expression of recombinant maize GSTUs (Lederer and Bger, 2005

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After centrifugation, 10 l of the supernatant were filtered through a Microcon YM-10 centrifugal filter device (Amicon), and analyzed by reversed-phase high-performance liquid chromatography on a C18 column (Atlantis 5 m, 4,6200 mm, Waters) as previously described [18]