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Description

Badens, C

This self-perpetuating loop drains the cells glutathione reserves, as the antioxidant is rapidly consumed in a desperate attempt to neutralize the surging ROS

N Engl J Med (2010) 362:167585

The beads were then washed 3 times with ice-cold 1 PBS and eluted with a buffer containing 20 mM glutathione, 100 mM TrisHCl (pH 8.0), 120 mM NaCl, and 10% glycerol