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As expected, incubation with only reduced MESNA (75 mM) yielded no active RNase A (Figure 5)

Samples were flash-frozen before later analysis

C57BL/6N mice with SIRT5 knockout (KO) were generated by knocking out Exon 3 on SIRT5 (starts from about 12.47% of the coding region and covers 14.41% of the coding region) using CRISPR/Cas9 technology (Fig

Therefore, the risk of inducing vascular pruning as opposed to normalization is minimal and it likewise may not be as susceptible to the same mechanisms of therapeutic resistance as other vasculature normalizing strategies