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doi:10.1146/annurev.pharmtox.45.120403.095857 [PubMed] 15

Briefly, cells were seeded onto 24-well chamber slides, rinsed with ice-cold PBS and methanol at 20 C for 10 min, and then permeabilized with 0.1% Triton X-100

We selected BA17 as the key brain region of interest to distinguish between AD patients with A only and those with both A aggregates and Tau hyperphosphorylation