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Using the dye accessibility assay, we found that UT3-induced ubiquitin unfolding was largely diminished if either the ridge or the cleft was mutated or blocked (L55A, E145K, or UT3-C19 fusion

Second, for patients with PI complicated by acute intestinal volvulus, the high risks of conservative treatment (such as intestinal necrosis and infection spread) must be fully communicated, and efforts should be made to perform surgery as early as possible to relieve obstruction