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The following formula was used: Monoamines level determination According to Ciarlone 59 , weighted samples of cortical tissue were added to cold acidified butanol (3 mL) in a small beaker and homogenized for 30 s before being placed in plastic tubes and centrifuged at 5000 rpm for 5 min

The precision results [expressed as total coefficients of variation (CV)] were as follows: 5.3%, 2.1% and 1.9% in plasma, and 4.5%, 1.6% and 3.1% in urine, for the low, medium and high QC samples, respectively

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HPLC analysis was performed to determine the concentrations of residual glucose and fructose, acetic acid, glycerol and ethanol when the fermentations had been terminated, as described by 4