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5 Implications and future perspectives of this study This study reveals that exposure to heavy metals such as Ni, Cu, and As induces specific disruptions in the respiratory microbiota of rat BALF, including the enrichment of Pseudomonas and Burkholderia and depletion of Ralstonia , which may act as early biomarkers of dysbiosis linked to lung dysfunction

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This led us to propose and develop a strategy that was first discovered in 1952 by Szybalski and Bryson 13

Aiming to overcome this challenge, in the present study, we further proposed a BBB-bindable nanoprobe with a decoration of the well-designed -glutamyl moiety that could be activated by the GGT enzyme overexpressed on BCECs