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Although ORD studies of polypeptides and proteins were conducted prior to CD, the dominance of ORD lasted only a few years, as in 1960, a French group presented an innovative approach to measure CD based on the use of a Pockels cell as an electro-optic retardation modulator (Grosjean and Legrand, 1960), which in combination with the adoption of the photoelastic modulator in 1966 ( An advantage of CD in the analysis of protein structure is determined by the ability of this technique to provide complementary structural information from different spectral regions (Kelly et al., 2005)