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doi: 10.1016/j.cub.2015.11.056 43 RiegerBThierbachSOmmerMDienhartFSFetznerSBuschKB

In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min