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After a cardiac origin is ruled out, the evaluation focuses on history, physical exam, and targeted testing

Inhibition of the Xc-system determines the decrease in GSH levels, which indirectly leads to the inactivation of GPX4 and the onset of ferroptosis ( 3+ enters cells with the assistance of transferrin and subsequently converts to Fe 2+ ( 2+ can catalyze the generation of metabolically toxic reactive oxygen species and phospholipid peroxide through the Fenton reaction, leading to cell damage or death (Figure 4)

It was observed that both FITC-HNG and FITC-SHLP3 were localized on the surface and inside the hair cells of gentamicin-exposed explants, while almost no labeling was found intracellularly in explants treated with FITC-MDPs alone