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Most studies have utilised analysis of DNA and/or RNA and/or protein on a biopsy from the primary tumour or less commonly, a metastatic lesion [2]

M.|McMillan, E.|Svendsen, C

Conditions appropriate for T cell culture include an appropriate media (e.g., Minimal Essential Media or RPMI Media 1640 or, X-vivo 15, (Lonza)) that may contain factors necessary for proliferation and viability, including serum (e.g., fetal bovine or human serum), interleukin-2 (IL-2), insulin, IFN- .gamma., IL-4, IL-7, GM-CSF, IL-10, IL-12, IL-15, TGF-, and TNF-a or any other additives for the growth of cells known to the skilled artisan