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(2015) Role of Pex21p for piggyback import of Gpd1p and Pnc1p into peroxisomes of Saccharomyces cerevisiae J

[11] For people struggling with a deficiency, a B12 supplement can support the relief of the signs and symptoms, some of which include: Pale skin Headaches Migraines Mood changes Although a vitamin B12 deficiency is more common in older adults, it's also often caused by insufficient dietary intake

Cells were collected by centrifugation (15 min, 4,500 g ) and resuspended in the cold lysis buffer containing 50 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF and 5 mM DTT