gst glutathione pdb PDB-101: Molecule of the Month: Transferases optimized to yield up to 10mg/250ml culture of soluble GST tagged protein The Multifaceted Role of Glutathione

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2012;14(5):535-U185

4.2.2 Cholesterol metabolism Cholesterol plays multiple vital roles in the body, including being a key component of cell membranes and plasma lipoproteins, as well as a precursor to steroidal hormones, bile acids, and oxysterols [133]

Tissues were homogenized in 510 mL of cold buffer (50 mM potassium phosphate, pH 7.5, 1 mM EDTA) per gram of tissue, followed by centrifugation at 10,000 g for 15 min at 4 C