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After blocking in 5% nonfat milk for 1 h at room temperature, the membrane was incubated sequentially with primary antibodies overnight at 4 C and HRP-linked secondary antibodies for 45 min at room temperature

Sulfide Oxidation Two copies of the gene for sulfide quinone reductase, sqr , are in the genome of Geitlerinema ( Supplementary Figure S4 )

MTs bind up to seven Zn(II) ions with varying affinities, from nano- to picomolar, playing a key role in zinc buffering and maintaining Zn(II) levels in mammalian cells (Kocya et al