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For strain preparation, cultures were centrifuged at 8000g for 3 min, washed in PBS (pH=7), and resuspended in the same buffer

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Recent research indicates that Spirulina supplementation might offer neuroprotective benefits and enhance cognitive performance in neurodegenerative disorders, including Alzheimer's and Parkinson's disease ( 3.7 The role of Spirulina in gut health Changes in intestinal microbiota and disruptions in gene expression and metabolic enzyme activity appear to negatively affect intestinal functions (104)

smegmatis cells were measured and calculated as described in Materials and Methods (Table 3)