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Using proteomic approaches, SIR has been identified as a TRX target in wheat seeds (Wong et al., 2004) and in Synechocystis (Wei et al., 2020)

The stage-tip was then washed four times with 150 l of 0.5% TFA/5% ACN (2,000 g , 2 min), and the sample was eluted using 100 l of 70% ACN/0.5% TFA (2,000 g , 3 min)