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The proteins were eluted with 250 mM imidazole in phosphate buffer

In this phase, studies have suggested that macrophages secrete proteases, especially matrix metalloproteinase-9 (MMP-9), to destroy elastin, fibrin, and other matrix proteins that the tension of the fibrous cap comes from, making macrophages an important player in plaque destabilization (97)

doi: 10.1080/10715762.2019.1688799 79

Abnormal serum bicarbonate levels should be investigated to determine if acidosis or alkalosis are present, as they can be associated with various health issues, including cardiovascular disease and diabetes