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The lysates were centrifuged at 4 C for 5 min, and supernatants were incubated with anti-SIRT1 (Ab110304, Abcam) or anti-SIRT3 (Ab246522, Abcam) antibodies for 4 h at 4 C

PLoS ONE 4 , e4715 (2009)

Quantitative PCR instrumentation was funded by Science Foundation Ireland (Grant no: SFI/07/RFP/GEN/F571/ECO7)

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