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For the ammonium chloride addition experiment, cells were dealt with 0.4 M NH 4 Cl for 72 h, and then the cells were collected for vacuolar staining

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Importantly, redox profiling also lends itself to integration with high-throughput omics, including proteomics, transcriptomics, and metabolomics, enabling multidimensional characterization of sperm dysfunction and the development of biomarker-driven therapeutic strategies