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Guide and passenger RNAs were each chemically synthesized (IDT) and gel-purified on a urea-polyacrylamide gel, then annealed into duplexes at 5 M final concentration in annealing buffer (30 mM Tris-HCl pH 7.5, 100 mM NaCl and 1 mM EDTA)

Transcriptional glucose signaling through the glucose response element is mediated by the pentose phosphate pathway

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Types of glaucoma Primary glaucomas show optic neuropathy in the presence or absence of elevated IOP (i.e., the cause of the disease is not known) whereas secondary glaucomas are defined by known pathological mechanisms that elevate IOP above normal