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Slides were deparaffinized in xylene, rehydrated through a graded ethanol series, and subjected to antigen retrieval in citrate buffer (pH 6.0) using microwave heating

f , HCC-1806 cells were treated with BSO (100 M) for 24 h, and relative GSH levels were determined

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Ethics declarations Ethics Approval All experiments involving animals were carried out in accordance with NIH guidelines and were approved by the University of Selcuk Animal Care and Use Committee (Protocol Number: 201828)