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In our study, environmental inflammatory factors including IL-1 were significantly increased in mice subjected to social isolation stress, which is in line with previous studies that indicated that people who experience adversity early in their lives have a significant increase in CRP and IL-6 levels compared to healthy individuals 43

doi: 10.3389/fnins.2023.1227705 11 MichinagaSKoyamaY

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For a more functional assessment, markers like methylmalonic acid (MMA) can be helpful, as they rise when B12 is functionally deficient even if serum levels are borderline