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The recommended concentration of clove oil (extracted from Syzygium aromaticum or Eugenia aromatica ) in handling processes is 1030 mgL 1 ( Sparus aurata ) at concentration of 44.5 LL 1 before air exposure for blood collection increased plasma cortisol and/or glucose values compared to control fish (handled as the anesthetized fish) ( 1 clove oil did not change plasma cortisol and glucose levels of the seawater meager manipulated for weighing compared to control fish (handled as the anesthetized fish) ( Solea senegalensis ) anesthesia with 1000 mgL 1 clove oil demonstrated a good ability of clove oil to prevent cortisol, lactate and glucose increases pre-mortem (Ribas et al., 2007)

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The total amount of -EC obtained by 5 times repeated reactions in the cellulose sponge was 305 mg, comparable with the 291 mg of MCC, which was more than three times higher than those of the filter paper used in our previous study under the same conditions (data not shown)