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the last washing supernatants of all processed biopsies and a mix of the acid + neutralizing buffer solutions utilized in the elution procedure were employed as internal negative controls (9)

1 Vannelli BG, Barni T, Orlando C, Natali A, Serio M, Balboni GC: Insulin-like growth factor-I (IGF-I) and IGF-I receptor in human testis: an immunohistochemical study