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This difference directly impacts how both proteins are purified from cell extracts, while Ts26GST is purified chromatographically with a glutathione Sepharose-4B affinity column, Ts24GST does not bind to this resin and must be purified by ion and filtration chromatographies

Together, these data suggest that ATF4 induction downstream of mTORC1 is necessary but not sufficient for mTORC1-regulated protein synthesis, consistent with the multiple mechanisms through which mTORC1 controls this key anabolic process

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